Specific and Acute Toxicity Estimate of New Domestic Nootropic Preparation

Author(s):

Kasimov Eldor*, Inoyatova Feruza, Azimova Sevara, Saydalikhodjaeva Ozoda, Boboeva Zukhra and Nasillayev Feruz

Purpose of the Research: To comparative specific properties and acute toxicity researching of a new domestic no otropic Nootrotem preparation. Materials and Methods: The research object was Nootrotem preparation, it is solution for infusion produced by LLC Temur Med Farm Uzbekistan and comparison preparations Mexidol and Piracetam. The preparation anti hypoxic activity was studied under model of normobaric hypoxic hypoxia in experiments on 18 white male mice, divided into 3 groups. They are Group 1 control to 0.9 percent, 0.3 ml volume NaCl solution were injected intravenously during 3 days, Group 2 experimental within 3 days no otrotem was injected at the dose of 337.5 mg by kg 0.3 ml, Group 3 comparison for 3 days, diluted comparison preparations Mexidol and Piracetam were injected intravenously at a dose of 337.5 mg by kg 0.3 ml. Acute toxicity was investigated by using a conventional method on white outbred male mice for heads. The research experiments were done in two stages. In the first stage, no otrotem was injected once into the tail vein of white mice as follows Group 1 6 mice to intravenous infusion at a dose of 112.5 mg by kg 0.1 ml, Group 2 6 mice to intravenous infusion at a dose of 337.5 mg by kg 0.3 ml; Group 3 6 mice to intravenous infusion at a dose of 562.5 mg by kg 0.5 ml. In the second stage experiment solution identical to the No otrotem preparation Mexidol 1 ampoule 5 ml plus Piracetam 1 ampoule 5 ml plus 0.9 percent NaCl, 90 ml was prepared from the preparations Mexidol and Piracetam, was administered once into the tail vein of white mice as follows Group 16 mice to intravenous infusion at a dose of 112.5 mg by kg 0.1 ml, Group 2 6 mice to intravenous infusion at a dose of 337.5 mg by kg 0.3 ml, Group 3 6 mice to intravenous infusion at a dose of 562.5 mg by kg 0.5 ml. After the completion of the experiment, the LD50 and the toxicity class of the preparations are determined. The data obtained were statistically processed using the STATISTICA program. Results: Nootrotem preparation on the model of normobaric hypoxic hypoxia increases the Resistance of mice to hypoxia: At a dose of 337.5 mg by kg it significantly lengthens the life span of animals by 23.6 percent, making 11.4 plus or minus 0.43 minutes, while as in the control group of mice, this indicator was 9.3 plus or minus 0.45 min. LD50 of Nootrotem and Mexidol plus Piracetam solution was greater thabn 562.5 mg by kg. Conclusion: Preparations have an equally reliable anti hypoxic effect and a low toxicological characteristic.