Casamadrid VRE

Department of Toxicology, Faculty of Chemistry, Autonomous University of the State of Mexico, (UAEMex), Mexico

Publications

• Original Article   
  Body Mass Index in Pregnancy Does Not Affect Peroxisome Proliferator‑activated Receptor Gamma Promoter Region (−359 to −260) Methylation in the Neonate
  Author(s): Casamadrid VRE, Amaya CA and Mendieta ZH*
  
  Background: Obesity in pregnancy can contribute to epigenetic changes. Aim: To assess whether body mass index (BMI) in pregnancy is associated with changes in the methylation of the peroxisome proliferatorâÃ?Â?Ã?Â?activated receptor γ (PPAR) promoter region (−359 to − 260) in maternal and neonatal leukocytes. Subjects and Methods: In this matched, cohort study 41 pregnant women were allocated into two groups: (a) Normal weight (n = 21) and (b) overweight (n = 20). DNA was extracted from maternal and neonatal leukocytes (4000–10,000 cells) in MagNA Pure (Roche) using MagNA Pure LC DNA Isolation Kit 1 (Roche, Germany). Treatment of DNA (2 μg) was performed with sodium bisulfite (EZ DNA MethylationâÃ?Â?Ã?Â?Direct™ Kit; Zymo Research). RealâÃ?Â?Ã?Â?time quantitative polymerase chain reaction (.. Read More»