Samuel Asamoah Sakyi

Department of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

Publications

• Original Article   
  Modified DNA Extraction Technique for Use in Resource-Limited Settings: Comparison of Salting Out Methods versus QIAamp Blood Mini Kit
  Author(s): Samuel Asamoah Sakyi*, Bright Kumi, Richard Dadzie Ephraim, Kwabena Owusu Danquah, Derrick Osakunor and David Baidoe-Ansah
  
  Background: It is essential that methods for genomic DNA extraction techniques produce high yield and purified DNA. Commercially available DNA extraction kits have taken over the traditional DNA extraction techniques. However, to meet the demands of cost-effectiveness, ready availability, safety, reliability and purity in resource-limited settings, an improved traditional DNA extraction method which meet the above criteria is required. Aim: We therefore evaluated the modified salting out and double salt precipitation method, against QIAamp Blood Mini Kit. Materials and Methods: In a cross-sectional study, DNA was extracted from venous blood of 60 suspected typhoid fever patients who visited the Komfo Anokye Teaching Hospital diagnostics department to do laboratory investigations that required blood collect.. Read More»